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Saturday 19 September 2009
Blue Square Conference North
(match 11)
at Corinium Stadium, Cirencester.
Blue Square North logo

New Tigers Badge: no-one knows why...

Gloucester City

1-3

Blyth Spartans

Blyth logo
 

HT (1-1)

 
 
Scorers:
W.Morford (16,72), L.Smith (59p)
City kit 07blyth kit colours
Scorers:
R.Dale (6)
         
 
M.Green
1.
C.Turns
J.Baldwin
2.
S.Harrison
L.Marshall
3.
K.Boyle
M.Richards
4.
M.Tait
T.Hamblin
5.
G.Williams ©
M.Rose
6.
D.Craddock
L.Smith
7.
J.Gillies
Man of the Match © T.Webb

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Metoclopramida 30 mg /kg/day, ip) and clomipramine 30 mg/kg/day, s.c.), were administered in the experimental group (n = 10) for 7 d. The control group (n = 8) received no treatment. In a separate session, the same rat was tested for spatial working memory by placing it in two identical chambers (60 × 20 10 cm) and recording each rat's location for 4 min and its response to the presence of a hidden platform (A). The response to a hidden platform was measured after 3 d. For spatial working memory, the following parameters were measured: total duration of the platform-induced response (3 d), latency from the platform position to first movement of the rat (10–20 s), and latency from the first Alprazolam cheapest price movement of rat to the disappearance platform (10–20 s). The animals in experimental group (n = 5) were Buy alprazolam online canada tested for a general spatial ability (n = 9) and for spatial working memory (n = 8) before and after treatment. In each experimental group, the animals were treated with following drugs Restoril 30mg 90 pills US$ 320.00 US$ 3.56 (20 mg/kg/day, ip) and received the following treatments (20 mg/kg/day, ip) 7 d apart: clomipramine 30 mg/kg/day, s.c.; quinpirole 2.5 mg/kg b.i.d., i.p.; sertraline 20–40 mg/kg/day, fluoxetine 10 mg/kg, p.o.; and restoril 15 mg pill citalopram p.o.): haloperidol 3 mg/kg/day, s.c. and imipramine 0.5 s.c.; 40 mg/kg/day, i.p. (n = 4); clonidine 5 mg/kg/day, s.c.; and prazosin 25–50 mg, best drugstore bb cream for acne skin i.p. (n = 3). At the end of experimental period, brain was snap-frozen in liquid nitrogen and stored at −80 °C until it was used for immunohistochemical detection of α 2-adrenoceptors. Brain specimens were processed to determine the levels of brain α 2-adrenoceptors by immunohistochemically staining hippocampal sections with anti-α 2-adrenoceptor antibody (1:200; Abcam), and the percentage of α 2-adrenoceptors was measured by immunohistochemical methods (see below). For the analysis of expression c-fos and N-methyl-d-aspartate (NMDA) receptors in the hippocampus and amygdala, rat brain tissue samples were homogenized and frozen in liquid nitrogen. The homogenates were then transferred to a microtiter plate and incubated in a buffer containing sodium orthovanadate (50 mM; Sigma Aldrich) and a protease inhibitor cocktail (50 mM tris-HCl, pH 8.3; 20 EGTA, 0.25% Triton X-100; 10 mM sodium orthovanadate; 1 SDS; phenylmethylsulfonyl fluoride; mM Na 2 EDTA; 150 NaCl; 0.1% sodium deoxycholate) at 4 °C overnight. After the incubation, protein extracts were incubated with an anti-c-fos antibody (1:1,000, Abcam) or an anti-NMDA antibody (1:1,000; Abcam) in a final volume of 1 ml for h at room temperature, followed by washing in tris (pH 8.0) pH 8.3 buffer for 2 min with a total of three subsequent washes in TBS, pH 8.0, at room temperature. The samples were then incubated with anti-rabbit Alexa Fluor 647-conjugated goat secondary antibody (1:1000; Vector Laboratories) at room temperature for 1 h. The samples were then washed in TBS, pH 8.0, several times and counterstained with hematoxylin eosin (Sigma) for visualization. The immunohistochemical analysis was performed as previously described [14]. In a further investigation based on our previous studies [13], [14], we tested the influence of treatment on expression NMDA and cFos receptors in the cortex of rat brain by in situ hybridization. tissue restoril 30 mg for sleep pill samples were digested in 1 h of trypsin (Roche Diagnostics, Mannheim, Germany) with protease inhibitors (100% ÎČ-mercaptoethanol, 1 mM dithiothreitol, MgCl 2 ).

S.Turnball
W.Morford
9.

P.Brayson

M.Symons
10.
I.Graham
J.Harris
11.
R.Dale
Subs
A.Sykes
12.
S.Todd (for 10, 76)
(for 10, 63) J.Pitcher
14.
J.Alexander (for 11, 76)
L.Ballinger
15.
J.Brackstone
J.Palmer
16.
-
A.Harris
17.
-
 
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
 
Bookings:
City: none
Blyth: none
Dismissals: none

T-Ender City MotM: Tom Webb - captain's performance from the new skipper, given planty of space to drive City forward from midfield.

grass trim rule

Finally, finally City laid to rest our new season home jinx at the sixth time of asking at the expense of a fragile looking Blyth side who faced a miserable long trip back to the North-East. City's attack made the most of the extra space they were allowed from the visitors, and despite riding our luck at time we were well worth a long-awaited home win. The only blot on an otherwise morale lifting display came with the sight of invaluable forward target man Mike Symons being carried off with an apparently serious ankle injury early in the second half.

City had Mike Green to thank for the match not getting off to a bad start, making a brave early save at the feet of Ian Graham as the Blyth striker found himself clean through on goal with the City defence evaporating. That should have served as a warning, but Spartans still grabbed an early lead a few minutes later. Our defensive concetration is still our achilles heel, and Hamblin gifted the ball straight to Spartans' danger man Robbie Dale on the edge of the area. His first effort was blocked by Green again, but the stranded keeper couldn't stop the second effort.

At least we'd made the mistake early, and had plenty of time to come back. The Spartans defence looked a little frail and they quickly found they couldn't manage Symons either in the air of if they gave him a chance to turn and run. His charge down the right flank looked to have run out of space but he managed to cut the ball back low across the penalty area. Smith reached it at the near post where his flick was only parried by the keeper, falling across an open goal towards Morf. The City striker has been a little low on confidence in front of goal, but he had time to take several touches and ponderously complete the tap in for his first of the campaign.

 

 

grass trim rule
T-Ender Match Snaps
Smith runs at Blyth Towering leap from Rico
Lee Smith gets a run at Spartans' Darren Craddock. Blyth keeper Turns just takes a cross away from Rico's path.
Jack Harris heads wide
Morf celebrates his second.
Jack Harris hangs in the air but his header flies wide. Morf and Pitch share a Spartans moment after the third goal.


Team Selection:

 

TBBM Said:

 

Tactics Board:
Anorak Corner:

 

T-Ender Verdict

 

Highlights Elsewhere:

 

Other Match Reports:

 

Further Reading:

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BBC Sport Online BSq North results page

Referee: Neil Radford (Worcester) City League Pos: 14th (+4) City Form: WLDDLW

Ref Watch:

Plenty of soft bookings and some inconsistent decisions (6) Attendance: 303    
Conditions: sunny intervals
Lorry Score:
2
Match Report:
by t-towel

Work commitments mean that we are keen to obtain other match reporters willing to provide details on some City matches. All text can be supplied by email, with no need to worry about web design or formatting. If you are interested please contact the T-Ender.

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